For routine formalin-fixed paraffin-embedded immunohistochemistry, 10% neutral buffered formalin is the standard starting fixative. It contains approximately 4% formaldehyde and provides a practical balance between tissue preservation and antigen detection....
Astor Scientific Team |
Endotoxin removal relies on the physicochemical differences between proteins and lipopolysaccharides (LPS). Key methods include Triton X-114 phase separation, anion-exchange chromatography (AEX), and polymyxin B affinity chromatography. The choice of...
Astor Scientific Team |
Western blot sample preparation is the critical first step that determines the success of the experiment. It involves lysing cells or tissues under cold conditions with suitable protease and phosphatase...
Astor Scientific Team |
Size exclusion chromatography (SEC) is a powerful laboratory technique that separates molecules in solution primarily according to their size, shape, and hydrodynamic radius. Unlike binding-based chromatography methods, SEC is designed...
Astor Scientific Team |
A biosafety cabinet and a laminar flow hood can look similar at first glance, but they are designed for very different laboratory goals. Both use controlled airflow and HEPA filtration...
Astor Scientific Team |
Collecting samples for laboratory testing is one of the most important steps in the diagnostic and research workflow. Before a sample reaches an analyzer, PCR platform, ELISA plate, microscope, centrifuge,...
Astor Scientific Team |
Pipette tips may look like simple disposable lab consumables, but they play a major role in research accuracy, contamination control, and workflow reliability. Whether a lab is preparing PCR master...
Astor Scientific Team |
Transport swabs and viral transport medium play a critical role in modern diagnostic testing, molecular biology, clinical laboratory testing, viral sample collection, and research workflows. When a specimen is collected...
Astor Scientific Team |
Serum-free media is an important option for researchers who want more control over cell culture conditions. In many traditional workflows, cell culture media is supplemented with animal serum, especially Fetal...
Astor Scientific Team |
MTT assay and trypan blue assay are two of the most common methods used to measure cell viability in cell culture, molecular biology, biotechnology, pharmaceutical research, cancer research, toxicology, and...
Astor Scientific Team |
Serum-free media has become an important option for researchers who want more control, consistency, and reproducibility in cell culture. Traditional cell growth media often contain Fetal Bovine Serum (FBS), which...
Astor Scientific Team |
DMEM, short for Dulbecco’s Modified Eagle Medium, is one of the most widely used basal media in mammalian cell culture. It supports the growth and maintenance of many adherent cell...
Astor Scientific Team |
Recombinant proteins are widely used in life science research, from ELISA standards and cell signaling studies to drug discovery, enzyme assays, and antibody development. For many laboratories, choosing the right...
Astor Scientific Team |