TribioScience’s Cell Counting Kit-8 (CCK-8) is a colorimetric assay to quantify the viable cells, consisting of the tetrazolium salt and the electron mediator 1-mthoxyPMS. The working principle is based on dehydrogenase activity detection. The generated NADH from the reaction of dehydrogenase enzymes and the substrates oxidizes the 1-methoxyPM. The reduced 1-methoxyPMS further oxidizes the tetrazolium salt (WST-8) in the CCK-8 assay kit, forming the yellow formazan dye. The dye color can then be quantified by measuring the absorbance at 460 nm wavelength so that the cell number is known. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.
Principle
Features
- Simplicity: One-bottle, ready-to-use solution. No harvesting, no washing, and no solubilization steps.
- Safety: No organic solvents or isotopes are required.
- Accuracy: Absorbance measurement is proportional to the number of cells in each well.
- Sensitivity: More sensitive than MTT, XTT, MTS, or WST-1.
Applications
- Cell proliferation
- Drug screening
- Growth factors
- Cytotoxicity
- Apoptosis
