Urea Colorimetric Assay

Description

Urea (carbamide, carbonyl diamide) is a predominant final metabolite of nitrogenous compounds in mammals, accounting for 80-90% of nitrogen excretion in humans and animals. Formed exclusively in the liver, urea is mostly transported by the bloodstream to the kidneys, where it is excreted into the urine. It is an indicator of liver and kidney functions. Determination of urea concentration is an essential task in clinical laboratories and research.
The Tribo™ Urea Colorimetric Assay provides a rapid, sensitive method to measure urea concentration in biological samples. The intensity of the color is directly proportional to the urea concentration in the sample. The assay can be performed in a convenient 96-well or 384-well microplate format and can be easily adapted to automation without a separation step.

Applications

• Direct Assay: of urea in serum, plasma, urine, milk, cell/tissue culture, animal products, and wine.
• Drug Discovery and Screening: effects of drugs on urea metabolism.

Key Features

• Sensitive and Accurate: The kit detects concentrations as low as 10µM in solution
• Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay

Storage Conditions

Store the kit at 4℃. For long-term storage, the kit should be stored at –20℃ protected from light.
Shelf life: 12 months.