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TribioScience  |  SKU: TBS2101

Tribo™ Tryptase Activity Assay

$280.00
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TribioScience

Tribo™ Tryptase Activity Assay

$280.00

Description

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules, comprising up to 20% of the total protein of mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.

The TribioTM Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for the evaluation of tryptase activity in cell lysates, supernatants, or for inhibitor screening.

Applications

  • Cell culture supernatants, cell lysates, or other tryptase-containing supplies.
  • Testing of purified tryptase enzyme, in vitro inhibitor screening.
  • Cell degranulation

Key Features

  • Sensitive and Accurate: The kit detects as low as 10μM in solution.
  • Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.

Storage Conditions:

Store the kit at -20℃, protect from light.
Shelf life: 6 months.

Standard Preparation (Fig.1)

Fig. 2: pNA Standard Curve

Fig. 3: Tryptase Positive Control Curve

Fig. 4: Effects of pH Value on Tryptase Activity

Size

  • 100 Tests
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