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Product Description
Description
Tribioscience’s Fast Mouse IL-1β ELISA kit is designed to quantitatively detect mouse IL-1β levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of mouse IL-1β samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural mouse IL-1β protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of mouse IL-1β is typically 15 pg/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant mouse IL-1β.
No cross-reactivity with others.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Mouse IL-1β Microplate
TBS3030A
96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for mouse IL-1β.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Mouse IL-1β
Standard
TBS3030B
20 µL of Recombinant mouse IL-1β (50 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3030C
2.1 mL of biotin-mouse IL-1β antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3030D
200 µL of streptavidin HRP.
Assay Diluent
TBS3030E
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Tribioscience’s Fast Mouse IL-1β ELISA kit is designed to quantitatively detect mouse IL-1β levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of mouse IL-1β samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural mouse IL-1β protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of mouse IL-1β is typically 15 pg/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant mouse IL-1β.
No cross-reactivity with others.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Mouse IL-1β Microplate
TBS3030A
96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for mouse IL-1β.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Mouse IL-1β
Standard
TBS3030B
20 µL of Recombinant mouse IL-1β (50 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3030C
2.1 mL of biotin-mouse IL-1β antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3030D
200 µL of streptavidin HRP.
Assay Diluent
TBS3030E
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
