Tribo™ Mouse CCL2/MCP-1 ELISA Kit

Description

Monocyte Chemotactic Protein-1 (MCP-1), also known as Chemokine (C-C motif) ligand 2 (CCL2), is a member of the beta (CC) subfamily of chemokines. MCP-1 is characterized as a monocyte-specific chemoattractant. Because of its target cell specificity, MCP-1 is postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally, it has been shown to chemoattract CD4+ and CD8+ T lymphocytes.

The Mouse MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse MCP-1 in different tissue plasma, serum, and other biological samples. The plate has been coated with murine MCP-1 monoclonal antibody. Any MCP-1 present in the sample or standard will be captured. A biotin-conjugated anti-murine MCP-1 antibody is added followed by streptavidin-horseradish peroxidase (HRP) and an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of MCP-1 in the initial sample. The reaction is terminated by the addition of a stop solution, and absorbance is measured at 450 nm. The concentration of MCP-1 in the samples is calculated opposite to the standard curve.
The mouse MCP-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Applications

Direct Assays: quantitative MCP-1 in plasma and serum.

Key Features

• Sensitivity: The minimum detectable dose of mouse MCP-1 is less than 8.0 pg/mL typically.
• Specificity: This assay recognizes recombinant and natural mouse MCP-1. No cross-reactivity with any of the other cytokine is observed.

Kit Contents

Storage Conditions

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receipt.