Red Blood Cell Lysis Kit – Enriched Single-Cell Prep

Overview

The The Red Blood Cell Lysis Kit is formulated for the rapid and effective removal of red blood cells (RBCs) from single-cell suspensions. Widely applied in flow cytometry, immunological studies, and molecular biology, this kit improves the clarity and quality of samples used for downstream cell analysis and extraction workflows.

Background Information

• Crucial for processing peripheral blood and lymphoid tissues.
• Enhances accuracy in:
  • Flow cytometry, by isolating white blood cells for phenotypic analysis.
  • Single-cell sequencing, by removing cellular noise caused by erythrocytes.
  • Protein and nucleic acid extraction, by providing purified cellular material.
• Widely used in clinical diagnostics, immunological research, and biomarker discovery.

Detection Principle

• Combines osmotic shock and enzymatic digestion:
  • The buffer induces a hypertonic environment that causes red blood cells to absorb water and rupture.
  • Enzymes degrade RBC-specific membrane components, ensuring selective lysis.
• Result:
  • White blood cells and other cell types remain intact, suitable for further analysis or functional assays.

Specifications

Kit Components