Product Name |
Recombinant HumanSAR1A Protein |
Product Overview |
This recombinant humanSAR1A protein includes amino acids 1-198aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
Target Uniprot Id |
Q9NR31 |
Recommended Name |
Small COPII coat GTPase SAR1A |
Gene Name |
SAR1A |
Synonyms |
SAR1a gene homolog 1, COPII-associated small GTPase, Masra2, SAR1, Sara, SARA1 |
Species |
Human |
Predicted Molecular Mass |
24.5 kDa |
Expression System |
E.coli |
Expression Range |
1-198aa |
Purity |
>90% |
Formulation |
Lyophilized |
Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
Research Area |
Signal Transduction |
Target Function |
Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). |
Subcellular Location |
Endoplasmic reticulum. Golgi apparatus. |
Protein Family |
Small GTPase superfamily, SAR1 family |