| Product Name |
Recombinant Mouse Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Protein |
| Product Overview |
This recombinant mouse Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) protein includes amino acids 19-352aa of the target gene is expressed in 293F cell.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P27467 |
| Recommended Name |
Protein Wnt-3a |
| Gene Name |
Wnt3a |
| Species |
Mouse |
| Predicted Molecular Mass |
65 kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
19-352aa |
| Tag |
C-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Developmental Biology |
| Target Function |
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro). |
| Subcellular Location |
Secreted, extracellular space, extracellular matrix. Secreted. |
| Protein Family |
Wnt family |
| Tissue Specificity |
Dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins. |
