| Description |
Recombinant Mouse WISP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr23-Asn367. |
| Purity |
> 95% as determined by Tris-Bis PAGE |
| Accession |
O54775 |
| Target Symbol |
WISP1 |
| Synonyms |
ELM-1; WISP-1; CCN4; WISP1c; WISP1i; WISP1tc |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Thr23-Asn367 |
| Mol. Weight |
The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
The interplay between glioma stem cells (GSCs) and the tumor microenvironment plays crucial roles in promoting malignant growth of glioblastoma (GBM), the most lethal brain tumor. WISP1 is preferentially expressed and secreted by GSCs. Silencing WISP1 markedly disrupts GSC maintenance, reduces tumor-supportive TAMs (M2), and potently inhibits GBM growth. WISP1 signals through Integrin α6β1-Akt to maintain GSCs by an autocrine mechanism and M2 TAMs through a paracrine manner. |
