| Description |
Recombinant Mouse Siglec-5/CD170 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr17-Leu439. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q920G3 |
| Target Symbol |
Siglec-5/CD170 |
| Synonyms |
CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2 |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Thr17-Leu439 |
| Mol. Weight |
The protein has a predicted MW of 46.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. |
