Description |
Recombinant Mouse RGM-C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln33-Asp393. |
Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Accession |
Q7TQ32 |
Target Symbol |
RGM-C |
Synonyms |
Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C |
Species |
Mouse |
Expression System |
HEK293 |
Tag |
C-His |
Expression Range |
Gln33-Asp393 |
Mol. Weight |
The protein has a predicted MW of 40 kDa (mature)&26 kDa (C-terminus peptide)&14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 55-60 kDa&36-40 kDa&20-25 kDa based on Tris-Bis PAGE result. |
Form |
Lyophilized |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping |
Shipped at ambient temperature. |
Gene Background |
RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system. |