| Description |
Recombinant Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
P06339(Q1-1b)&P01887(B2M)&AMAPRTLLL |
| Target Symbol |
Q1-1b&B2M&Qdm (AMAPRTLLL) Monomer |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His-Avi |
| Expression Range |
His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide |
| Mol. Weight |
The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Qa-1b binds a peptide (AMAPRTLLL), referred to as Qdm (for Qa-1 determinant modifier), derived from the signal sequence of murine class Ia molecules. This peptide binds with high affinity and accounts for almost all of the peptides associated with this molecule. Human histocompatibility leukocyte antigen (HLA)-E, a homologue of Qa-1b, binds similar peptides derived from human class Ia molecules and interacts with CD94/NKG2 receptors on natural killer cells. |
