| Description |
Recombinant Mouse LRRN1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser26-Ala631. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q61809 |
| Target Symbol |
LRRN1 |
| Synonyms |
Leucine-rich repeat neuronal protein 1; Neuronal leucine-rich repeat protein 1; NLRR-1; NLRR1; FIGLER3; KIAA1497; LRN |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Ser26-Ala631 |
| Mol. Weight |
The protein has a predicted MW of 69.28 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Lrrn1 is required for the formation of MHB--loss of function leads to a loss of the morphological constriction and loss of Fgf8. Cells overexpressing Lrrn1 violate the boundary and result in a loss of cell restriction between midbrain and hindbrain compartments. Lrrn1 also regulates the glycosyltransferase Lunatic Fringe, a modulator of Notch signalling, maintaining its expression in midbrain cells which is instrumental in MHB boundary formation. |
