| Description |
Recombinant Mouse LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu438. |
| Purity |
> 95% as determined by Tris-Bis PAGE |
| Accession |
P16301 |
| Target Symbol |
LCAT |
| Synonyms |
PAF acetylhydrolase; LCAT |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Phe25-Glu438 |
| Mol. Weight |
The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 67-75 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport. |
