| Description |
Recombinant Mouse GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Pro327. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q00941 |
| Target Symbol |
GM-CSF R alpha |
| Synonyms |
GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4 |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Leu30-Pro327 |
| Mol. Weight |
The protein has a predicted MW of 33.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Mouse GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA (QC Test). The affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. |
