| Description |
Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr40-Arg217. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
NP_001070657.1 |
| Target Symbol |
Fc gamma RIIB/CD32b |
| Synonyms |
Fc gamma Receptor IIB/C; CD32; CD32b; CDw32; FCGR2B/C; CD32b/c; FcRII-b/c; FCG2; IGFR2; CD32A; Fc gamma RII; Fc gamma RIIB; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Thr40-Arg217 |
| Mol. Weight |
The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
The affinity constant of 0.280 uM as determined in SPR assay (Biacore T200). Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens. |
