| Description |
Recombinant Mouse ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala112-Gly224. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q9Z0L2 |
| Target Symbol |
ARTN |
| Synonyms |
Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
N-hFc |
| Expression Range |
Ala112-Gly224 |
| Mol. Weight |
The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in 50mM Glycine,150mM NaCl (pH 3.2). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 32.2ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons. |
