| Description |
Recombinant Mouse AMIGO2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met39-Thr397. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q80ZD9 |
| Target Symbol |
AMIGO2 |
| Synonyms |
Amphotein-induced protein 2; AMIGO-2; Alivin-1; DEGA; AMIGO2; ALI1; alivin 1 |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Met39-Thr397 |
| Mol. Weight |
The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Bromodomain and extraterminal domain inhibitors (BETi) represent promising therapeutic agents for metastatic melanoma, yet their mechanism of action remains unclear. Here we interrogated the transcriptional effects of BETi and identified AMIGO2, a transmembrane molecule, as a BET target gene essential for melanoma cell survival. AMIGO2 is upregulated in melanoma cells and tissues compared to human melanocytes and nevi, and AMIGO2 silencing in melanoma cells induces G1/S arrest followed by apoptosis. |
