| Product Name |
Recombinant Human Zinc Finger BED Domain-Containing 1/ZBED1 Protein |
| Product Overview |
This recombinant human Zinc Finger BED Domain-Containing 1/ZBED1 protein includes amino acids 3-100aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
O96006 |
| Recommended Name |
E3 SUMO-protein ligase ZBED1 |
| Gene Name |
ZBED1 |
| Synonyms |
Zinc Finger BED Domain-Containing Protein 1; Putative Ac-Like Transposable Element; dREF Homolog; ZB |
| Species |
Human |
| Predicted Molecular Mass |
12.5 kDa |
| Expression System |
E.coli |
| Expression Range |
3-100aa |
| Tag |
C-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA. This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12. The resulting increased transcriptional activity drives cell proliferation. Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins.; (Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection. |
| Subcellular Location |
Nucleus, PML body. Nucleus.; Nucleus, PML body. |
| Tissue Specificity |
Ubiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta. |
