| Product Name |
Recombinant Human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Protein |
| Product Overview |
This recombinant human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) protein includes amino acids 1-290aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P56704 |
| Recommended Name |
Protein Wnt-3a |
| Gene Name |
WNT3A |
| Synonyms |
Protein Wnt-3a, wnt-3a |
| Species |
Human |
| Predicted Molecular Mass |
41 kDa |
| Expression System |
E.coli |
| Expression Range |
1-290aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction, Developmental Biology |
| Target Function |
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro). |
| Subcellular Location |
Secreted, extracellular space, extracellular matrix. Secreted. |
| Protein Family |
Wnt family |
| Tissue Specificity |
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. |
