| Product Name |
Recombinant Human Tryptophanyl tRNA Synthetase (WARS/WARS1) Protein |
| Product Overview |
This recombinant human Tryptophanyl tRNA Synthetase (WARS/WARS1) protein includes amino acids 247-458aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P23381 |
| Recommended Name |
Tryptophan--tRNA ligase, cytoplasmic |
| Gene Name |
WARS1 |
| Synonyms |
GAMMA-2, IFI53, IFP53, Tryptophanyl-tRNA synthetase, cytoplasmic, TrpRS, hWRS |
| Species |
Human |
| Predicted Molecular Mass |
30 kDa |
| Expression System |
E.coli |
| Expression Range |
247-458aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. |
| Subcellular Location |
Cytoplasm. |
| Protein Family |
Class-I aminoacyl-tRNA synthetase family |
