| Product Name |
Recombinant Human Vimentin (VIM) Protein |
| Product Overview |
This recombinant human Vimentin (VIM) protein includes amino acids 2-466aa of the target gene is expressed in E.coli.The protein is supplied in liquid or lyophilized powder form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P08670 |
| Recommended Name |
Vimentin |
| Gene Name |
VIM |
| Species |
Human |
| Predicted Molecular Mass |
55.0 kDa |
| Expression System |
E.coli |
| Expression Range |
2-466aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Liquid or Lyophilized powder |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Subcellular Location |
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane. |
| Protein Family |
Intermediate filament family |
| Associated Diseases |
Cataract 30, multiple types (CTRCT30) |
| Tissue Specificity |
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
