| Product Name |
Recombinant Human U2AF1 Protein |
| Product Overview |
This recombinant human U2AF1 protein includes amino acids 1-240aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q01081 |
| Recommended Name |
Splicing factor U2AF 35 kDa subunit |
| Gene Name |
U2AF1 |
| Synonyms |
U2 small nuclear RNA auxiliary factor 1, FP793, RN, RNU2AF1, Splicing factor U2AF 35 kDa subunit, U2 |
| Species |
Human |
| Predicted Molecular Mass |
30.3 kDa |
| Expression System |
E.coli |
| Expression Range |
1-240aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. |
| Subcellular Location |
Nucleus. Nucleus speckle. |
| Protein Family |
Splicing factor SR family |
| Associated Diseases |
Myelodysplastic syndrome (MDS) |
