| Product Name |
Recombinant Human Thioredoxin Reductase 1 (TXNRD1) Protein |
| Product Overview |
This recombinant human Thioredoxin Reductase 1 (TXNRD1) protein includes amino acids 412-647aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q16881 |
| Recommended Name |
Thioredoxin reductase 1, cytoplasmic |
| Gene Name |
TXNRD1 |
| Synonyms |
GRIM-12, MGC9145, TR, TR1, TRXR1, TXNR, Thioredoxin reductase 1 Gene associated with retinoid IFN in |
| Species |
Human |
| Predicted Molecular Mass |
32 kDa |
| Expression System |
E.coli |
| Expression Range |
412-647aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology |
| Target Function |
Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. |
| Subcellular Location |
Cytoplasm.; [Isoform 4]: Cytoplasm. Nucleus.; [Isoform 5]: Cytoplasm. |
| Protein Family |
Class-I pyridine nucleotide-disulfide oxidoreductase family |
| Tissue Specificity |
Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, |
