| Product Name |
Recombinant Human Thiopurine Methyltransferase (TPMT) Protein |
| Product Overview |
This recombinant human Thiopurine Methyltransferase (TPMT) protein includes amino acids 26-227aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P51580 |
| Recommended Name |
Thiopurine S-methyltransferase |
| Gene Name |
TPMT |
| Synonyms |
Thiopurine S-methyltransferase, TPMT, Thiopurine S-methyltransferase HGNC:12014, S adenosyl L methio |
| Species |
Human |
| Predicted Molecular Mass |
27 kDa |
| Expression System |
E.coli |
| Expression Range |
26-227aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism |
| Target Function |
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor. TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified. |
| Subcellular Location |
Cytoplasm. |
| Protein Family |
Class I-like SAM-binding methyltransferase superfamily, TPMT family |
