| Product Name |
Recombinant Human Thrombomodulin (TM/THBD) Protein |
| Product Overview |
This recombinant human Thrombomodulin (TM/THBD) protein includes amino acids 325-481aa of the target gene is expressed in E.coli.The protein is supplied in liquid or lyophilized powder form and formulated in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.prior to lyophilization. |
| Target Uniprot Id |
P07204 |
| Recommended Name |
Thrombomodulin |
| Gene Name |
THBD |
| Synonyms |
CD141; THBD; BDCA3; THRM; Fetomodulin |
| Species |
Human |
| Predicted Molecular Mass |
25 kDa |
| Expression System |
E.coli |
| Expression Range |
325-481aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Liquid or Lyophilized powder |
| Buffer |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cardiovascular |
| Target Function |
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. |
| Subcellular Location |
Membrane; Single-pass type I membrane protein. |
| Associated Diseases |
Thrombophilia due to thrombomodulin defect (THPH12); Hemolytic uremic syndrome atypical 6 (AHUS6) |
| Tissue Specificity |
Endothelial cells are unique in synthesizing thrombomodulin. |
