| Product Name |
Recombinant Human Transcription factor 7-like 2 (TCF7L2) Protein |
| Product Overview |
This recombinant human Transcription factor 7-like 2 (TCF7L2) protein includes amino acids 1-355 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
Q9NQB0 |
| Recommended Name |
Transcription factor 7-like 2 |
| Gene Name |
TCF7L2 |
| Synonyms |
TCF7L2, TCF4 |
| Species |
Human |
| Predicted Molecular Mass |
42 kDa |
| Expression System |
E.coli |
| Expression Range |
1-355 |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. |
| Subcellular Location |
Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies. |
| Protein Family |
TCF/LEF family |
| Associated Diseases |
Diabetes mellitus, non-insulin-dependent (NIDDM) |
| Tissue Specificity |
Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived |
