| Product Name |
Recombinant Human SNAP-25 (Synaptosomal-associated 25kDa/SNAP25) Protein |
| Product Overview |
This recombinant human SNAP-25 (Synaptosomal-associated 25kDa/SNAP25) protein includes amino acids 1-206 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in 20mM Tris-HCl pH7.5, 50mM NaCl, 5mMDTT, 1mM EDTA, 10%Glycerolprior to lyophilization. |
| Target Uniprot Id |
P60880 |
| Recommended Name |
Synaptosomal-associated protein 25 |
| Gene Name |
SNAP25 |
| Synonyms |
SNAP25, SNAP-25, Synaptosomal-associated protein 25kDa, FLJ23079, RIC-4, RIC4, SEC9, SNAP 25, bA416N4.2, dJ1068F16.2, SUP, resistance to inhibitors of cholinesterase 4 homolog, super protein, synaptosomal-associated 25 kDa protein |
| Species |
Human |
| Predicted Molecular Mass |
23 kDa (207 aa) |
| Expression System |
E.coli |
| Expression Range |
1-206 |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
20mM Tris-HCl pH7.5, 50mM NaCl, 5mMDTT, 1mM EDTA, 10%Glycerol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Neuroscience |
| Target Function |
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. |
| Subcellular Location |
Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Photoreceptor inner segment. |
| Protein Family |
SNAP-25 family |
| Associated Diseases |
Myasthenic syndrome, congenital, 18 (CMS18) |
| Tissue Specificity |
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. |
