| Product Name |
Recombinant Human SMYD3 / ZMYND1 Protein |
| Product Overview |
This recombinant human SMYD3 / ZMYND1 protein includes amino acids 35-369aa of the target gene is expressed in Baculovirus-Insect Cells.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9H7B4 |
| Recommended Name |
Histone-lysine N-methyltransferase SMYD3 |
| Gene Name |
SMYD3 |
| Synonyms |
bA74P14.1;KMT3E;ZMYND1;ZNFN3A1 |
| Species |
Human |
| Predicted Molecular Mass |
656 kDa |
| Expression System |
Insect Cell |
| Expression Range |
35-369aa |
| Tag |
N-GST |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Protein Family |
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family |
| Tissue Specificity |
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. |
