| Product Name |
Recombinant Human Mothers Against Decapentaplegic Homolog 2 (Smad2) Protein |
| Product Overview |
This recombinant human Mothers Against Decapentaplegic Homolog 2 (Smad2) protein includes amino acids 274-467 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in 20mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 20% glycerolprior to lyophilization. |
| Target Uniprot Id |
Q15796 |
| Recommended Name |
Mothers against decapentaplegic homolog 2 |
| Gene Name |
SMAD2 |
| Synonyms |
Sma- and Mad-related protein 2 isoform 1, hMAD-2, hSMAD2, JV18, JV18-1, MADH2, MADR2, Sma- and Mad-related protein 2 isoform 1 JV181, MAD, MAD Related Protein 2, SMAD2. |
| Species |
Human |
| Predicted Molecular Mass |
26kDa |
| Expression System |
E.coli |
| Expression Range |
274-467 |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 20% glycerol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Target Function |
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Protein Family |
Dwarfin/SMAD family |
| Tissue Specificity |
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. |
