| Product Name |
Recombinant Human Excitatory Amino Acid Transporter 3 (EAAT3/SLC1A1) Protein |
| Product Overview |
This recombinant human Excitatory Amino Acid Transporter 3 (EAAT3/SLC1A1) protein includes amino acids 115-209aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P43005 |
| Recommended Name |
Excitatory amino acid transporter 3 |
| Gene Name |
SLC1A1 |
| Synonyms |
SLC1A1; EAAC1; Solute Carrier 1 Member 1; Sodium-dependent glutamate/aspartate transporter 3; Excita |
| Species |
Human |
| Predicted Molecular Mass |
14 kDa |
| Expression System |
E.coli |
| Expression Range |
115-209aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Neuroscience |
| Target Function |
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport. Negatively regulated by ARL6IP5. |
| Subcellular Location |
Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. Early endosome membrane. Late endosome membrane. Recycling endosome membrane. |
| Protein Family |
Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A1 subfamily |
| Associated Diseases |
Dicarboxylic aminoaciduria (DCBXA); Schizophrenia 18 (SCZD18) |
| Tissue Specificity |
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). |
