| Description |
Recombinant Human SEMA3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn21-Val771. |
| Purity |
> 95% as determined by Tris-Bis PAGE |
| Accession |
Q14563 |
| Target Symbol |
SEMA3A |
| Synonyms |
Semaphorin-3A; Semaphorin III; Sema III; Semaphorin-D; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL; semaphorin L |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
N-His |
| Expression Range |
Asn21-Val771 |
| Mol. Weight |
The protein has a predicted MW of 87.7 kDa. Due to cleavage site, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Anti-SEMA3A Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30ug/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain. |
