| Product Name |
Recombinant Human RRM2B / P53R2 Protein |
| Product Overview |
This recombinant human RRM2B / P53R2 protein includes amino acids 1-351aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q7LG56 |
| Recommended Name |
Ribonucleoside-diphosphate reductase subunit M2 B |
| Gene Name |
RRM2B |
| Synonyms |
MTDPS8A;MTDPS8B;P53R2 |
| Species |
Human |
| Predicted Molecular Mass |
42.6 kDa |
| Expression System |
E.coli |
| Expression Range |
1-351aa |
| Tag |
N-His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. |
| Subcellular Location |
Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus in response to DNA damage. |
| Protein Family |
Ribonucleoside diphosphate reductase small chain family |
| Associated Diseases |
Mitochondrial DNA depletion syndrome 8A (MTDPS8A); Mitochondrial DNA depletion syndrome 8B (MTDPS8B); Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 5 (PEOA5) |
| Tissue Specificity |
Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. |
