| Product Name |
Recombinant Human Ribonuclease T2 (RNASET2) Protein |
| Product Overview |
This recombinant human Ribonuclease T2 (RNASET2) protein includes amino acids 25-256aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
O00584 |
| Recommended Name |
Ribonuclease T2 |
| Gene Name |
RNASET2 |
| Synonyms |
Rnase-T2; RNASE6PL; RNASE6-PL; Ribonuclease 6 |
| Species |
Human |
| Predicted Molecular Mass |
57 kDa |
| Expression System |
E.coli |
| Expression Range |
25-256aa |
| Tag |
N-His-GST |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Ribonuclease that plays an essential role in innate immune response by recognizing and degrading RNAs from microbial pathogens that are subsequently sensed by TLR8. Cleaves preferentially single-stranded RNA molecules between purine and uridine residues, which critically contributes to the supply of catabolic uridine and the generation of purine-2',3'-cyclophosphate-terminated oligoribonucleotides. In turn, RNase T2 degradation products promote the RNA-dependent activation of TLR8. Plays also a key role in degradation of mitochondrial RNA and processing of non-coding RNA imported from the cytosol into mitochondria. Participates as well in degradation of mitochondrion-associated cytosolic rRNAs. |
| Subcellular Location |
Secreted. Lysosome lumen. Endoplasmic reticulum lumen. Mitochondrion intermembrane space. |
| Protein Family |
RNase T2 family |
| Associated Diseases |
Leukoencephalopathy, cystic, without megalencephaly (LCWM) |
| Tissue Specificity |
Ubiquitous. Higher expression levels observed in the temporal lobe and fetal brain. |
