| Product Name |
Recombinant Human Rhodopsin (RHO) Protein |
| Product Overview |
This recombinant human Rhodopsin (RHO) protein includes amino acids 1-110aa of the target gene is expressed in E.coli.The protein is supplied in liquid or lyophilized powder form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P08100 |
| Recommended Name |
Rhodopsin |
| Gene Name |
RHO |
| Synonyms |
OPN2; RP4; Opsin 2; Rod Pigment; Retinitis Pigmentosa 4; Visual Purple |
| Species |
Human |
| Predicted Molecular Mass |
42.4 kDa |
| Expression System |
E.coli |
| Expression Range |
1-110aa |
| Tag |
N-His-GST |
| Purity |
>95% |
| Formulation |
Liquid or Lyophilized powder |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling. |
| Subcellular Location |
Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. |
| Protein Family |
G-protein coupled receptor 1 family, Opsin subfamily |
| Associated Diseases |
Retinitis pigmentosa 4 (RP4); Night blindness, congenital stationary, autosomal dominant 1 (CSNBAD1) |
| Tissue Specificity |
Rod shaped photoreceptor cells which mediate vision in dim light. |
