| Product Name |
Recombinant Human Rho-related GTP-binding RhoD (RhoD) Protein |
| Product Overview |
This recombinant human Rho-related GTP-binding RhoD (RhoD) protein includes amino acids 18-207aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
O00212 |
| Recommended Name |
Rho-related GTP-binding protein RhoD |
| Gene Name |
RHOD |
| Synonyms |
Rho-related GTP-binding protein RhoD, ARHD,RHOHP1, RHOM |
| Species |
Human |
| Predicted Molecular Mass |
23.8 kDa |
| Expression System |
E.coli |
| Expression Range |
18-207aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution. |
| Subcellular Location |
Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome. |
| Protein Family |
Small GTPase superfamily, Rho family |
| Tissue Specificity |
Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues. |
