| Product Name |
Recombinant Human Prothrombin (F2) Protein |
| Product Overview |
This recombinant human Prothrombin (F2) protein includes amino acids 1-622 of the target gene is expressed in 293F cell.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
P00734 |
| Recommended Name |
Prothrombin |
| Gene Name |
F2 |
| Synonyms |
Coagulation factor II, F2, Prothrombin, PT, Thrombin |
| Species |
Human |
| Predicted Molecular Mass |
70 kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
1-622 |
| Tag |
No |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cardiovascular |
| Target Function |
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. |
| Subcellular Location |
Secreted, extracellular space. |
| Protein Family |
Peptidase S1 family |
| Associated Diseases |
Factor II deficiency (FA2D); Ischemic stroke (ISCHSTR); Thrombophilia due to thrombin defect (THPH1); Pregnancy loss, recurrent, 2 (RPRGL2) |
| Tissue Specificity |
Expressed by the liver and secreted in plasma. |
