| Product Name |
Recombinant Human Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) Protein |
| Product Overview |
This recombinant human Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) protein includes amino acids 2-382aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P35813 |
| Recommended Name |
Protein phosphatase 1A |
| Gene Name |
PPM1A |
| Synonyms |
Protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform, PPM1A, PP2CA, PP2C-ALPHA |
| Species |
Human |
| Predicted Molecular Mass |
46.6 kDa |
| Expression System |
E.coli |
| Expression Range |
2-382aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. |
| Subcellular Location |
Nucleus. Cytoplasm, cytosol. Membrane; Lipid-anchor. |
| Protein Family |
PP2C family |
