| Product Name |
Recombinant Human Peroxisome Proliferator Activated Receptor Alpha (PPARa) Protein |
| Product Overview |
This recombinant human Peroxisome Proliferator Activated Receptor Alpha (PPARa) protein includes amino acids 61-170aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
Q07869 |
| Recommended Name |
Peroxisome proliferator-activated receptor alpha |
| Gene Name |
PPARA |
| Synonyms |
NR1C1; PPAR-A; HPPAR; Nuclear Receptor Subfamily 1 Group C Member 1 |
| Species |
Human |
| Predicted Molecular Mass |
18 kDa |
| Expression System |
E.coli |
| Expression Range |
61-170aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. |
| Subcellular Location |
Nucleus. |
| Protein Family |
Nuclear hormone receptor family, NR1 subfamily |
| Tissue Specificity |
Skeletal muscle, liver, heart and kidney. Expressed in monocytes. |
