| Product Name |
Recombinant Human Octamer Binding Transcription Factor 4 (OCT4/POU5F1) Protein |
| Product Overview |
This recombinant human Octamer Binding Transcription Factor 4 (OCT4/POU5F1) protein includes amino acids 136-360aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q01860 |
| Recommended Name |
POU domain, class 5, transcription factor 1 |
| Gene Name |
POU5F1 |
| Synonyms |
POU domain, class 5, transcription factor 1 isoform 2, POU5F1, MGC22487, OCT3, OCT4, OTF3, OTF4 |
| Species |
Human |
| Predicted Molecular Mass |
29 kDa |
| Expression System |
E.coli |
| Expression Range |
136-360aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Protein Family |
POU transcription factor family, Class-5 subfamily |
| Tissue Specificity |
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. |
