| Product Name |
Recombinant Human Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3) Protein |
| Product Overview |
This recombinant human Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3) protein includes amino acids 647-738aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
O60568 |
| Recommended Name |
Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3 |
| Gene Name |
PLOD3 |
| Synonyms |
LH3; Lysyl hydroxylase 3 |
| Species |
Human |
| Predicted Molecular Mass |
14 kDa |
| Expression System |
E.coli |
| Expression Range |
647-738aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Others |
| Target Function |
Multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine. Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues. Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues. Essential for normal biosynthesis and secretion of type IV collagens. Essential for normal formation of basement membranes. |
| Subcellular Location |
Rough endoplasmic reticulum. Endoplasmic reticulum lumen. Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side. Secreted. Secreted, extracellular space. |
| Associated Diseases |
Lysyl hydroxylase 3 deficiency (LH3 deficiency) |
| Tissue Specificity |
Ubiquitous. Detected in heart, placenta and pancreas and at lower levels in lung, liver and skeletal muscle. |
