| Product Name |
Recombinant Human Plasminogen (Plg) Protein |
| Product Overview |
This recombinant human Plasminogen (Plg) protein includes amino acids 274-560aa of the target gene is expressed in E.coli.The protein is supplied in liquid or lyophilized powder form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P00747 |
| Recommended Name |
Plasminogen |
| Gene Name |
PLG |
| Synonyms |
PL; Plasmin; Activation peptide; Angiostatin |
| Species |
Human |
| Predicted Molecular Mass |
33.5 kDa |
| Expression System |
E.coli |
| Expression Range |
274-560aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Liquid or Lyophilized powder |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
SDS-PAGE; WB; ELISA; IP |
| Research Area |
Cardiovascular, Others, Cancer |
| Target Function |
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.; Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. |
| Subcellular Location |
Secreted. Note=Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. |
| Protein Family |
Peptidase S1 family, Plasminogen subfamily |
| Associated Diseases |
Plasminogen deficiency (PLGD) |
| Tissue Specificity |
Present in plasma and many other extracellular fluids. It is synthesized in the liver. |
