| Product Name |
Recombinant Human Phytanoyl-CoA dioxygenase, peroxisomal(PHYH) Protein |
| Product Overview |
This recombinant human Phytanoyl-CoA dioxygenase, peroxisomal(PHYH) protein includes amino acids 31-338aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
O14832 |
| Recommended Name |
Phytanoyl-CoA dioxygenase, peroxisomal |
| Gene Name |
PHYH |
| Synonyms |
LN1;LNAP1;PAHX;PHYH1;RD |
| Species |
Human |
| Predicted Molecular Mass |
35.6 kDa |
| Expression System |
E.coli |
| Expression Range |
31-338aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Catalyzes the 2-hydroxylation of not only racemic phytanoyl-CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms). Does not hydroxylate long and very long straight chain acyl-CoAs or 2-methyl- and 4-methyl-branched acyl-CoAs. |
| Subcellular Location |
Peroxisome. |
| Protein Family |
PhyH family |
| Associated Diseases |
Refsum disease (RD) |
| Tissue Specificity |
Expressed in liver, kidney, and T-cells, but not in spleen, brain, heart, lung and skeletal muscle. |
