| Product Name |
Recombinant Human Perforin 1 (PRF1) Protein |
| Product Overview |
This recombinant human Perforin 1 (PRF1) protein includes amino acids 32-316aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P14222 |
| Recommended Name |
Perforin-1 |
| Gene Name |
PRF1 |
| Synonyms |
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein |
| Species |
Human |
| Predicted Molecular Mass |
62 kDa |
| Expression System |
E.coli |
| Expression Range |
32-316aa |
| Tag |
N-His-GST |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Immunology |
| Target Function |
Pore-forming protein that plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. |
| Subcellular Location |
Cytolytic granule. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. |
| Protein Family |
Complement C6/C7/C8/C9 family |
| Associated Diseases |
Familial hemophagocytic lymphohistiocytosis 2 (FHL2) |
