| Product Name |
Recombinant Human Pyruvate dehydrogenase alpha 1 (PDHA1) Protein |
| Product Overview |
This recombinant human Pyruvate dehydrogenase alpha 1 (PDHA1) protein includes amino acids 30-390aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P08559 |
| Recommended Name |
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
| Gene Name |
PDHA1 |
| Synonyms |
PDHCE1A; PHE1A; PHE1-A; PDHa; PDH-A; Pyruvate Dehydrogenase(lipoamide)Alpha 1; Pyruvate Dehydrogenas |
| Species |
Human |
| Predicted Molecular Mass |
46 kDa |
| Expression System |
E.coli |
| Expression Range |
30-390aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism |
| Target Function |
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Subcellular Location |
Mitochondrion matrix. |
| Associated Diseases |
Pyruvate dehydrogenase E1-alpha deficiency (PDHAD) |
| Tissue Specificity |
Ubiquitous. |