| Product Name |
Recombinant Human Platelet Derived Growth Factor Subunit B (PDGFB) Protein |
| Product Overview |
This recombinant human Platelet Derived Growth Factor Subunit B (PDGFB) protein includes amino acids 21-241aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P01127 |
| Recommended Name |
Platelet-derived growth factor subunit B |
| Gene Name |
PDGFB |
| Synonyms |
Platelet-derived growth factor subunit B isoform 2, c-sis, IBGC5, PDGF-2, PDGF2, SIS, SSV |
| Species |
Human |
| Predicted Molecular Mass |
33 kDa |
| Expression System |
E.coli |
| Expression Range |
21-241aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. |
| Subcellular Location |
Secreted. Note=Released by platelets upon wounding. |
| Protein Family |
PDGF/VEGF growth factor family |
| Associated Diseases |
Basal ganglia calcification, idiopathic, 5 (IBGC5) |
| Tissue Specificity |
Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. |
