| Product Name |
Recombinant Human Pterin-4-α-Carbinolamine Dehydratase/PHS/PCBD1 Protein |
| Product Overview |
This recombinant human Pterin-4-α-Carbinolamine Dehydratase/PHS/PCBD1 protein includes amino acids 2-104aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P61457 |
| Recommended Name |
Pterin-4-alpha-carbinolamine dehydratase |
| Gene Name |
PCBD1 |
| Synonyms |
Pterin-4-Alpha-Carbinolamine Dehydratase; PHS; 4-Alpha-Hydroxy-Tetrahydropterin Dehydratase; Dimeriz |
| Species |
Human |
| Predicted Molecular Mass |
14.2 kDa |
| Expression System |
E.coli |
| Expression Range |
2-104aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling, Metabolism |
| Target Function |
Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity. Also acts as a coactivator for HNF1B-dependent transcription. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Protein Family |
Pterin-4-alpha-carbinolamine dehydratase family |
| Associated Diseases |
Hyperphenylalaninemia, BH4-deficient, D (HPABH4D) |
