| Product Name |
Recombinant Human PDK / PDZ binding kinase / TOPK (PBK) Protein |
| Product Overview |
This recombinant human PDK / PDZ binding kinase / TOPK (PBK) protein includes amino acids 1-322aa of the target gene is expressed in Baculovirus-Insect Cells.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q96KB5 |
| Recommended Name |
Lymphokine-activated killer T-cell-originated protein kinase |
| Gene Name |
PBK |
| Synonyms |
CT84;HEL164;Nori-3;SPK;TOPK |
| Species |
Human |
| Predicted Molecular Mass |
37 kDa |
| Expression System |
Insect Cell |
| Expression Range |
1-322aa |
| Tag |
C-His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology, Signal Transduction |
| Target Function |
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. |
| Protein Family |
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily |
| Tissue Specificity |
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. |
