| Product Name |
Recombinant Human PARP2 Protein |
| Product Overview |
This recombinant human PARP2 protein includes amino acids 233-583aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9UGN5 |
| Recommended Name |
Poly [ADP-ribose] polymerase 2 |
| Gene Name |
PARP2 |
| Synonyms |
Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2 |
| Species |
Human |
| Predicted Molecular Mass |
42.5 kDa |
| Expression System |
E.coli |
| Expression Range |
233-583aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex. |
| Subcellular Location |
Nucleus. Chromosome. |
| Tissue Specificity |
Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine |
![SDS-PAGE analysis of Human PARP2 (Poly [Adp-Ribose] Polymerase 2) - Recombinant Protein, showing >85% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1gs112pok11vk1qu3j.jpg?v=1755755139&width=1000)
![SDS-PAGE analysis of Human PARP2 (Poly [Adp-Ribose] Polymerase 2) - Recombinant Protein, showing >85% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1gs112pok11vk1qu3j.jpg?v=1755755139&width=88)
![SDS-PAGE analysis of Human PARP2 (Poly [Adp-Ribose] Polymerase 2) - Recombinant Protein, showing >85% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1gs112pok11vk1qu3j.jpg?v=1755755139&width=640)
